CANDIDA ALBICANS IN GUT ENHANCED THE SEVERITY OF DEXTRAN SULFATE SOLUTION-INDUCED COLITIS MOUSE MODEL

  • Pratsanee Hiengrach
  • Asada Leelahavanichkul
  • Ariya Chindamporn
Keywords: Colitis, Candida albicans, Gut translocation, Dextran sulfate sodium

Abstract

The influence of gut fungi against acute colitis is intriguing. While, dextran sulfate sodium (DSS)-induced colitis mouse model is a representative model for colitis in patients, Candida albicans, are detectable only in human intestine, but not in mouse. Thus, 106 cells/ml of C. albicans were administered in 3% DSS-colitis mouse model to explore the influence of the fungi toward the model.

DSS-C. albicans administration enhanced the severity of colitis model as determined by the increased serum IL-6 cytokine level (a pro-inflammatory cytokine).The additive effect of heat-kill C. albicans and Escherichia coli toward HT 29 cell, a human colon adenocarcinoma cell line, in the production of IL-8 cytokine, neutrophil chemotactic factor, compared to the cells activated with E. coli or C. albicans alone were also demonstrated.

In conclusion, C. albicans enhanced the severity and the inflammatory response in DSS induced colitis mouse model.

Published
2018-09-01