SELECTION OF CYTOKINE - INDUCED KILLER CELLS ON AUTOIMMUNE HEPATITIS IN MOUSE

Abstract

Autoimmune hepatitis (AIH) is a chronic inflammatory disease of liver that the pathogenic mechanisms of AIH have not yet been clarified. All patients with AIH lead to cirrhosis and liver cancer. Presently the best feasible medicines of AIH need aid immunosuppressive medications and liver transplantation which have many side effects, high cost and sustained remission. Thus, immunotherapy is an alternative therapy in which cellular material is injected into a patient. Many previous studies showed cytokine induced killer (CIK) cells, T lymphocytes that have a phenotype of NK cells, have a potential to against several diseases associated with liver. The current research aims to examine protective effects of CIK cells on AIH in mice.  CIK cells cultured from thymus and spleen were then used to develope a protocol for generating CIK cells by adding interferon gamma (IFN-g), monoclonal antibody (mAb) against CD3 on day 0  and interleukin-2 (IL-2) every 2 or 3 day then flow cytometry were used to analyze CIK cells. The result suggest that cell from thymus were suitable for this study.