EFFECTS OF PLATELET-RICH FIBRIN ON THE PROLIFERATION AND CALCIFICATION OF MICE OSTEOBLASTS IN VITRO

Abstract

The ultimate goal of osseous defect treatment is regeneration of bone resorbed, for example, by tooth loss. This study aimed to investigate the effect of platelet-rich fibrin (PRF) on proliferation and calcification of osteoblast cell line MC3T3-E1 in vitro. Osteoblast cells were exposed to PRF at various concentrations (6.25 to 50 percent) for a period of 1 and 7 days wherein the viability of osteoblast cells was assessed by Prestoblue assay. Osteoblast cells not exposed to PRF served as comparison control. Results show that PRF concentration of 50 percent had the highest effect on increased osteoblast cells proliferation for a period of 1 day and PRF concentration of 6.25 percent had the highest effect on increased osteoblast cells proliferation for a period of 1 day. Furthermore, the concentration of 6.25 percent had the highest effect on increased calcification. In conclusion, PRF was effective in stimulating osteoblast proliferation and calcification in vitro.